Vaishali Shah
Associate professor, VIVA Institute of Pharmacy, India
Title: DEVELOPMENT AND EVALUATION OF POLYHERBAL FORMULATION FOR UROLITHIASIS
Biography
Biography: Vaishali Shah
Abstract
Urolithiasis, one of the most painful ailments of the urinary tract disorder, has beset humans from centuries. Medical treatments for kidney stone are relatively costly, painful and require expert hands and availability of appropriate equipments. So, the present research work had been undertaken for the Development and evaluation of polyherbal formulation for kidney stone. The formulation was developed using powders of Tribulus terrestris, Crataeva nurvala, Kalanchoe pinnata which are individually well reported in Ayurveda for Urolithiassis. Morphological, microscopical study and physicochemical parameters of all the plants present in formulations were done to identify the plants. The phytochemical screening was performed for presence of phytoconstituents. Total phenolic & total flavanoids content were calculated by Folin ciocalteau method and AlCl3 colorimetric method. Antioxidant activity was done by DPPH assay, Superoxide free radical scavenging activity & Reducing power assay. Anti-lithiatic acivity was performed by In vitro model- homogenous precipitation method. Phytochemical screening of formulation showed the presence of alkaloids, tannins, saponins, flavonoid. Total phenol and flavonoid content was 0.53 % and 0.51 % w/w respectively. It showed significant in vitro antioxidant activity in dose dependent manner as evident by antioxidant assay. Anti-lithiatic activity was performed which shows that the formulation has good anti-lithiatic activity and supports its tradional uses for kidney stone and showed synergistic effect in combination